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SiRNA Validation Data

siRNA ID:

143280

Gene ID:

5257

Transcript(s):

NM_000293.2 NM_001031835.2 XM_005255983.4 XM_005255984.4 XM_017023283.1 XM_017023284.1

Validation Results:

11%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176028_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.