SiRNA Validation Data

siRNA ID:

15469

Gene ID:

Transcript(s):

NM_001013406.1 NM_004912.3 NM_194454.1 NM_194455.1 NM_194456.1 XM_005250660.3 XM_005250662.3 XM_005250665.3 XM_005250666.3 XM_005250667.2 XM_005250668.3 XM_005250669.3 XM_006716161.3 XM_006716162.3 XM_006716163.3 XM_011516651.2 XM_011516653.2 XM_011516654.2 XM_011516655.2 XM_011516656.2 XM_011516657.2 XM_011516658.2 XM_011516659.2 XM_011516660.2 XM_011516661.2 XM_017012755.1 XM_017012756.1 XM_017012757.1 XM_017012758.1 XM_017012759.1

Validation Results:

9%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00184988_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.