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SiRNA Validation Data

siRNA ID:

286

Gene ID:

5315

Transcript(s):

NM_001206796.2 NM_001206798.2 NM_001206799.1 NM_001316318.1 NM_002654.5 NM_182470.3 NM_182471.3 XM_005254443.1 XM_005254445.4 XM_006720570.1 XM_011521670.2 XM_017022313.1

Validation Results:

10%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.