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SiRNA Validation Data

siRNA ID:

369

Gene ID:

6733

Transcript(s):

NM_001278273.1 NM_182691.2 NM_182692.2 XM_005250549.3 XM_005250550.3 XM_005250551.4 XM_006716098.2 XM_011516536.2 XM_011516538.2 XM_017012560.1 XM_017012561.1 XM_017012562.1 XM_017012563.1 XM_017012564.1 XM_017012565.1 XM_017012566.1

Validation Results:

11%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.