Close X

SiRNA Validation Data

siRNA ID:

812

Gene ID:

10494

Transcript(s):

NM_001271977.1 NM_001271978.1 NM_001282305.1 NM_001282306.1 NM_001282307.1 NM_001282308.1 NM_006374.4 XM_011510493.2 XM_011510494.2 XM_011510495.2 XM_011510496.2 XM_017003168.1 XM_017003169.1 XM_017003170.1 XM_017003171.1

Validation Results:

24%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.