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SiRNA Validation Data

siRNA ID:

s10575

Gene ID:

5315

Transcript(s):

NM_001206796.2 NM_001206797.2 NM_001206798.2 NM_001206799.1 NM_001316318.1 NM_002654.5 NM_182470.3 NM_182471.3 XM_005254443.1 XM_005254445.4 XM_006720570.1 XM_011521670.2 XM_017022313.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00762869_s1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.