SiRNA Validation Data

siRNA ID:

s11631

Gene ID:

5830

Transcript(s):

NM_000319.4 NM_001131023.1 NM_001131024.1 NM_001131025.1 NM_001131026.1 NM_001300789.1 XM_005253455.1 XM_011520793.2 XM_011520795.1 XM_011520797.1 XM_011520798.1 XM_011520799.2 XM_011520800.1 XM_011520801.2 XM_011520802.2 XM_017019744.1 XM_017019745.1 XM_017019746.1 XM_017019747.1 XM_017019748.1 XM_017019749.1 XM_017019750.1 XM_017019751.1 XM_017019752.1 XM_017019753.1 XM_017019754.1 XM_017019755.1 XM_017019756.1 XM_017019757.1

Validation Results:

15%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00165604_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.