SiRNA Validation Data

siRNA ID:

s19746

Gene ID:

10142

Transcript(s):

NM_005751.4 NM_147185.2 XM_017011642.1 XM_017011643.1 XM_017011644.1 XM_017011645.1 XM_017011646.1 XM_017011647.1 XM_017011648.1 XM_017011649.1 XM_017011650.1 XM_017011651.1 XM_017011652.1 XM_017011653.1 XM_017011654.1 XM_017011655.1 XM_017011656.1 XM_017011657.1

Validation Results:

16%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00323977_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.