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SiRNA Validation Data

siRNA ID:

s20570

Gene ID:

10494

Transcript(s):

NM_001271977.1 NM_001271978.1 NM_001271979.1 NM_001271980.1 NM_001282305.1 NM_001282306.1 NM_001282307.1 NM_001282308.1 NM_006374.4 XM_011510493.2 XM_011510494.2 XM_011510495.2 XM_011510496.2 XM_017003168.1 XM_017003169.1 XM_017003170.1 XM_017003171.1

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00179039_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.