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SiRNA Validation Data

siRNA ID:

s23843

Gene ID:

23443

Transcript(s):

NM_001271684.1 NM_001271685.1 NM_012243.2 XM_005270691.4 XM_011541136.2 XM_011541137.2 XM_011541138.2 XM_017000870.1

Validation Results:

14%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00608439_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.