SiRNA Validation Data

siRNA ID:

s26154

Gene ID:

27327

Transcript(s):

NM_014494.2 XM_005255254.3 XM_005255257.4 XM_017023143.1 XM_017023144.1 XM_017023145.1 XM_017023146.1 XM_017023147.1 XM_017023148.1 XM_017023149.1 XM_017023150.1 XM_017023151.1 XM_017023152.1 XM_017023153.1 XM_017023154.1

Validation Results:

20%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00325721_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.