SiRNA Validation Data

siRNA ID:

s2757

Gene ID:

995

Transcript(s):

NM_001287582.1 NM_001287583.1 NM_001318098.1 NM_001790.4 NM_022809.3 XM_005272145.3 XM_011543759.2 XM_011543760.2 XM_011543761.2 XM_011543762.1 XM_011543764.1 XM_017010101.1

Validation Results:

14%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00156411_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.