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SiRNA Validation Data

siRNA ID:

s38

Gene ID:

163

Transcript(s):

NM_001030006.1 NM_001282.2 XM_005257937.3 XM_005257938.2 XM_011524448.1 XM_011524449.2 XM_011524450.1 XM_011524451.1 XM_011524452.1 XM_011524453.1 XM_011524454.1 XM_011524455.2 XM_017024284.1 XM_017024285.1 XM_017024286.1

Validation Results:

13%(+/-11% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00426694_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.