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SiRNA Validation Data

siRNA ID:

s390

Gene ID:

11269

Transcript(s):

NM_001014449.2 NM_001014451.2 NM_001257172.1 NM_001257173.1 NM_001257174.1 NM_001257175.1 NM_007242.5 XM_006721127.2 XM_011522832.2 XM_011522833.2 XM_011522834.2 XM_017022890.1

Validation Results:

12%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00246678_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.