SiRNA Validation Data

siRNA ID:

s41248

Gene ID:

94081

Transcript(s):

NM_001322977.1 NM_001322978.1 NM_001322980.1 NM_001322981.1 NM_001322982.1 NM_001322983.1 NM_022754.6

Validation Results:

8%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00224259_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.