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SiRNA Validation Data

siRNA ID:

s5785

Gene ID:

2710

Transcript(s):

NM_000167.5 NM_001128127.2 NM_001205019.1 NM_203391.3 XM_006724483.2 XM_006724484.2 XM_006724485.2 XM_011545491.2 XM_011545492.1 XM_017029409.1 XM_017029411.1

Validation Results:

4%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs02340011_g1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.