SiRNA Validation Data

siRNA ID:

s7551

Gene ID:

3679

Transcript(s):

NM_002206.2 XM_005268839.1 XM_005268840.1 XM_005268841.1 XM_005268842.1 XM_005268844.1 XM_005268846.1 XM_005268848.1 XM_005268849.1 XM_005268850.1 XM_017019265.1

Validation Results:

8%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00174397_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.