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SiRNA Validation Data

siRNA ID:

s8959

Gene ID:

4361

Transcript(s):

NM_005590.3 NM_005591.3 XM_005274008.3 XM_006718842.3 XM_011542837.2 XM_017017772.1 XM_017017773.1

Validation Results:

15%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00271551_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.