SiRNA Validation Data

siRNA ID:

2799

Gene ID:

4311

Transcript(s):

NM_000902.3 NM_007287.2 NM_007288.2 NM_007289.2 XM_006713646.3 XM_006713647.3 XM_011512855.2 XM_011512856.2 XM_011512857.2

Validation Results:

17%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00153510_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.