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SiRNA Validation Data

siRNA ID:

4535

Gene ID:

7182

Transcript(s):

NM_001291694.1 NM_003298.4 XM_011534058.2 XM_011534059.2 XM_011534061.2 XM_011534063.2 XM_011534064.2 XM_011534065.2 XM_011534066.2 XM_017007118.1 XM_017007119.1 XM_017007120.1

Validation Results:

27%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.