SiRNA Validation Data

siRNA ID:

s12822

Gene ID:

6470

Transcript(s):

NM_001281786.1 NM_004169.4 NM_148918.2 XM_005256767.3 XM_011523992.2 XM_017024957.1 XM_017024958.1 XM_017024959.1 XM_017024960.1

Validation Results:

12%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00541038_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.