Close X

SiRNA Validation Data

siRNA ID:

s223114

Gene ID:

100287932 100652748

Transcript(s):

NM_006327.3 NR_073029.1 XM_011539098.1 XM_011539099.1 NM_001290117.1 NM_001290118.1 NR_110767.1 NR_135475.1 XM_011539104.1 XM_017015460.1 XM_017015461.1 XM_017015462.1

Validation Results:

15%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00197056_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.