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SiRNA Validation Data

siRNA ID:

s271

Gene ID:

6873

Transcript(s):

NM_003184.3 XM_006716621.3 XM_011517259.2 XM_017013779.1 XM_017013780.1 XM_017013781.1 XM_017013782.1 XM_017013783.1 XM_017013784.1 XM_017013785.1 XM_017013786.1 XM_017013787.1 XM_017013788.1 XM_017013789.1 XM_017013790.1 XM_017013791.1

Validation Results:

18%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00162527_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.