SiRNA Validation Data

siRNA ID:

s28636

Gene ID:

51763

Transcript(s):

NM_001135642.1 NM_016532.3 NM_130766.2 XM_005256683.2 XM_005256685.1 XM_005256686.1 XM_011523934.1 XM_011523936.2 XM_017024755.1 XM_017024756.1 XM_017024757.1 XM_017024758.1 XM_017024759.1

Validation Results:

20%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00213017_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.