SiRNA Validation Data

siRNA ID:

s7452

Gene ID:

Transcript(s):

NM_001134224.1 NM_001134225.1 NM_001566.2 NM_004027.2 XM_006712499.3 XM_006712500.3 XM_006712501.3 XM_006712502.3 XM_006712503.3 XM_006712504.3 XM_006712505.3 XM_006712506.3 XM_006712507.3 XM_006712508.3 XM_011511122.2 XM_011511123.2 XM_011511124.2 XM_011511125.2 XM_017003993.1 XM_017003994.1 XM_017003995.1 XM_017003996.1 XM_017003997.1 XM_017003998.1 XM_017003999.1 XM_017004000.1 XM_017004001.1 XM_017004002.1 XM_017004003.1

Validation Results:

11%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00244467_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.