SiRNA Validation Data

siRNA ID:

121353

Gene ID:

Transcript(s):

NM_001286708.1 NM_001286710.1 NM_001286711.1 NM_001286712.1 NM_001995.3 XM_005262828.1 XM_005262829.1 XM_005262831.1 XM_011531742.1 XM_017007887.1 XM_017007888.1 XM_017007889.1

Validation Results:

10%(+/-11% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00242530_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.