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SiRNA Validation Data

siRNA ID:

1474

Gene ID:

132

Transcript(s):

NM_001123.3 NM_001202450.1 NM_006721.3 XM_017015698.1 XM_017015700.1 XM_017015701.1 XM_017015702.1 XM_017015704.1 XM_017015705.1 XM_017015706.1

Validation Results:

5%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00417073_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.