Close X

SiRNA Validation Data

siRNA ID:

s10689

Gene ID:

5358

Transcript(s):

NM_001136025.4 NM_001172335.2 NM_001282337.1 NM_001282338.1 NM_005032.6 XM_011537534.1

Validation Results:

4%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00192406_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.