SiRNA Validation Data

siRNA ID:

s195011

Gene ID:

10018

Transcript(s):

NM_001204108.1 NM_001204109.1 NM_001204111.1 NM_001204112.1 NM_001204113.1 NM_006538.4 NM_138621.4 NM_138622.3 NM_138623.3 NM_138624.3 NM_138626.3 NM_207002.3 XM_005263550.3 XM_005263551.2 XM_005263552.3 XM_005263553.2 XM_005263554.2 XM_005263556.3 XM_005263557.4 XM_005263561.3 XM_011510461.2 XM_011510464.2 XM_017003101.1

Validation Results:

11%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00197982_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.