SiRNA Validation Data

siRNA ID:

s28484

Gene ID:

51657

Transcript(s):

NM_001317785.1 NM_001317786.1 NM_001317787.1 NM_001317788.1 NM_001317789.1 NM_016086.2 NR_134486.1 NR_134487.1 XM_011516292.2 XM_011516293.2 XM_011516300.1 XM_017012296.1 XM_017012297.1 XM_017012298.1 XM_017012299.1 XM_017012300.1 XM_017012301.1 XM_017012302.1

Validation Results:

7%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00211467_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.