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SiRNA Validation Data

siRNA ID:

s406

Gene ID:

57038

Transcript(s):

NM_001318785.1 NM_020320.4 NR_134857.1 XM_011535948.2 XM_011535949.2 XM_017011072.1 XM_017011073.1 XM_017011074.1 XM_017011075.1 XM_017011076.1 XM_017011077.1 XM_017011078.1 XM_017011079.1 XM_017011080.1 XM_017011081.1

Validation Results:

17%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00368084_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.