SiRNA Validation Data

siRNA ID:

s4995

Gene ID:

2180

Transcript(s):

NM_001286708.1 NM_001286710.1 NM_001286711.1 NM_001286712.1 NM_001995.3 XM_005262828.1 XM_005262829.1 XM_005262831.1 XM_011531742.1 XM_017007887.1 XM_017007888.1 XM_017007889.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00242530_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.