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SiRNA Validation Data

siRNA ID:

s809

Gene ID:

781

Transcript(s):

NM_000722.3 XM_005250570.2 XM_005250572.2 XM_005250573.2 XM_005250574.2 XM_006716118.2 XM_006716119.3 XM_006716120.3 XM_011516570.2 XM_011516571.2 XM_011516572.2 XM_017012588.1

Validation Results:

9%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00167808_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.