SiRNA Validation Data

siRNA ID:

s11577

Gene ID:

5802

Transcript(s):

NM_002850.3 NM_130853.2 NM_130854.2 NM_130855.2 XM_005259600.2 XM_005259606.2 XM_005259607.2 XM_005259609.1 XM_005259610.1 XM_011528157.2 XM_011528158.2 XM_017027065.1 XM_017027066.1 XM_017027067.1 XM_017027068.1 XM_017027069.1 XM_017027070.1 XM_017027071.1 XM_017027072.1 XM_017027073.1 XM_017027074.1 XM_017027075.1 XM_017027076.1

Validation Results:

18%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00370080_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.