Close X

SiRNA Validation Data

siRNA ID:

121549

Gene ID:

6386

Transcript(s):

NM_001007067.1 NM_001007068.1 NM_001007069.1 NM_001007070.1 NM_005625.3 XM_011517571.2 XM_017013727.1 XM_017013728.1

Validation Results:

3%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs01045460_g1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.