4140231
NM_001128918.2 NM_001128919.2 NM_001128920.2 NM_001128921.2 NM_002376.6 XM_005267641.4 XM_005267642.4 XM_006720146.3 XM_011536769.2 XM_017021291.1 XM_017021292.1 XM_017021293.1 XM_017021294.1 XM_017021295.1 XM_017021296.1 XM_017021297.1 XM_017021298.1 XM_017021299.1 XM_017021300.1 XM_017021301.1 XM_017021302.1 XM_017021303.1 XM_017021304.1 XM_017021305.1 XM_017021306.1 XM_017021307.1 XM_017021308.1 XM_017021309.1 XM_017021310.1 XM_017021311.1
9%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.