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SiRNA Validation Data

siRNA ID:

s1500

Gene ID:

351

Transcript(s):

NM_000484.3 NM_001136016.3 NM_001136129.2 NM_001136130.2 NM_001136131.2 NM_001204301.1 NM_001204302.1 NM_001204303.1 NM_201413.2 NM_201414.2

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00169098_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.