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SiRNA Validation Data

siRNA ID:

s16158

Gene ID:

8505

Transcript(s):

NM_001303486.1 NM_001303487.1 NM_001303489.1 NM_001324381.1 NM_003631.3 NR_130168.1 NR_130169.1 NR_136752.1 NR_136753.1 NR_136754.1 NR_136755.1 NR_136756.1 XM_011540305.1

Validation Results:

17%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00608254_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.