SiRNA Validation Data

siRNA ID:

s21678

Gene ID:

11011

Transcript(s):

NM_001284333.1 NM_001284363.1 NM_006852.3 XM_011524215.2 XM_011524216.2 XM_011524217.2 XM_011524218.2 XM_011524220.2 XM_011524221.2 XM_011524222.2 XM_011524223.2 XM_011524224.2 XM_011524225.2 XM_011524226.2 XM_011524227.2 XM_011524228.2 XM_011524229.2 XM_017024044.1 XM_017024045.1 XM_017024046.1 XM_017024047.1 XM_017024048.1 XM_017024049.1 XM_017024050.1 XM_017024051.1 XM_017024052.1 XM_017024053.1 XM_017024054.1 XM_017024055.1 XM_017024056.1 XM_017024057.1 XM_017024058.1 XM_017024059.1 XM_017024060.1

Validation Results:

20%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00851784_g1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.