SiRNA Validation Data

siRNA ID:

s9788

Gene ID:

4931

Transcript(s):

NM_001243146.1 NM_001243147.1 NM_002533.3 NM_206840.2 XM_011544196.1 XM_011544198.1 XM_011544199.1 XM_011544200.1 XM_017001378.1 XM_017001379.1 XM_017001380.1 XM_017001381.1 XM_017001382.1 XM_017001383.1 XM_017001384.1 XM_017001386.1

Validation Results:

15%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00159705_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.