SiRNA Validation Data

siRNA ID:

120597

Gene ID:

Transcript(s):

NM_001166418.1 NM_001166419.1 NM_001166420.1 NM_001166422.1 NM_001166448.1 NM_018486.2 NR_051952.1 XM_011530986.2 XM_011530987.2 XM_017029640.1 XM_017029641.1 XM_017029642.1 XM_017029643.1 XM_017029644.1 XM_017029645.1 XM_017029646.1 XM_017029647.1

Validation Results:

16%(+/-11% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00218503_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.