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SiRNA Validation Data

siRNA ID:

1477

Gene ID:

11200

Transcript(s):

NM_001005735.1 NM_001257387.1 NM_007194.3 NM_145862.2 XM_011529839.2 XM_011529840.2 XM_011529841.1 XM_011529842.2 XM_011529843.1 XM_011529844.2 XM_017028560.1

Validation Results:

19%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.