SiRNA Validation Data

siRNA ID:

1651

Gene ID:

4363

Transcript(s):

NM_004996.3 XM_011522497.1 XM_011522498.2 XM_017023237.1 XM_017023238.1 XM_017023239.1 XM_017023240.1 XM_017023241.1 XM_017023242.1 XM_017023243.1

Validation Results:

15%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.