Close X

SiRNA Validation Data

siRNA ID:

142901

Gene ID:

6502

Transcript(s):

NM_005983.3 NM_032637.3 XM_011514081.2 XM_011514082.2 XM_011514083.2 XM_017009753.1

Validation Results:

4%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00180634_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.