SiRNA Validation Data

siRNA ID:

s34963

Gene ID:

64794

Transcript(s):

NM_001322340.1 NM_001322341.1 NM_001322342.1 NM_001322343.1 NM_001322344.1 NM_022779.8 NM_138620.1 NR_136309.1 XM_005272207.4 XM_006717236.3 XM_011518921.2 XM_011518922.2

Validation Results:

18%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00224384_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.