SiRNA Validation Data

siRNA ID:

103342

Gene ID:

Transcript(s):

NM_001284333.1 NM_001284363.1 NM_006852.3 XM_011524216.2 XM_011524217.2 XM_011524221.2 XM_011524222.2 XM_011524224.2 XM_011524225.2 XM_011524226.2 XM_011524227.2 XM_011524228.2 XM_011524229.2 XM_011524231.1 XM_017024044.1 XM_017024045.1 XM_017024046.1 XM_017024047.1 XM_017024048.1 XM_017024049.1 XM_017024051.1 XM_017024052.1 XM_017024053.1 XM_017024056.1 XM_017024057.1 XM_017024058.1 XM_017024059.1

Validation Results:

14%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00851784_g1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.