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SiRNA Validation Data

siRNA ID:

4237

Gene ID:

5029

Transcript(s):

NM_002564.3 NM_176071.2 NM_176072.2 XM_005274019.4 XM_005274020.4 XM_005274021.4 XM_011545074.2 XM_017017839.1

Validation Results:

16%(+/-6% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00175732_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.