SiRNA Validation Data

siRNA ID:

s10111

Gene ID:

5099

Transcript(s):

NM_001173523.1 NM_002589.2 NM_032456.2 NM_032457.3 XM_005248163.3 XM_005248164.3 XM_005248166.3 XM_011513842.2 XM_011513843.2 XM_011513844.2 XM_017008259.1 XM_017008260.1 XM_017008261.1 XM_017008262.1 XM_017008263.1 XM_017008264.1 XM_017008265.1 XM_017008266.1 XM_017008267.1 XM_017008268.1 XM_017008269.1 XM_017008270.1 XM_017008271.1 XM_017008272.1

Validation Results:

10%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00159908_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.