Close X

SiRNA Validation Data

siRNA ID:

s13568

Gene ID:

6788

Transcript(s):

NM_001256312.1 NM_001256313.1 NM_006281.3 XM_011517248.2 XM_011517251.1 XM_011517252.2 XM_011517257.2 XM_017013756.1 XM_017013757.1 XM_017013758.1 XM_017013759.1 XM_017013760.1 XM_017013761.1 XM_017013762.1

Validation Results:

15%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00169491_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.