SiRNA Validation Data

siRNA ID:

s2755

Gene ID:

994

Transcript(s):

NM_001287516.1 NM_001287517.1 NM_001287518.1 NM_001287519.1 NM_001287520.1 NM_001287522.1 NM_001287524.1 NM_004358.4 NM_021872.3 NM_021873.3 NR_136335.1 NR_136336.1

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00244740_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.